Transient expression of chicken alpha interferon gene in lettuce
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چکیده
منابع مشابه
CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI
The plasmid pALCA1SIFN containing cDNA that encodes the human interferon a-2b was obtained from the ATCC(no. 531667). In this system the expression of the gene is under the control of an alcA promoter. alcA p is a specific promoter for expression of different genes in Aspergillusfilamentous. In this plasmid the coding region of IFN?-2b is preceded by the coding region of a synthetic signal ...
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vp2 protein is the major host protective immunogen of infectious bursal disease virus (ibdv) of chickens. plants are now gaining widespread acceptance as a general platform for the large scale production of recombinant proteins including vaccine, antibodies and pharmaceuticals. in present study vp2 gene of an iranian isolate of ibdv was cloned under strong plant expression promoter camv35s and ...
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Interferon gamma (IFN-γ) is one of the key cytokines in defining T helper 1 lymphocyte immuneresponses. In this study, the bovine IFN-γ gene was cloned from spleen tissue RNA using the reversetranscription-polymerase chain reaction (RT-PCR). IFN-γ cDNA was sub-cloned and expressed inmammalian expression plasmid (pcDNA3.1(+)) under the control of the human cytomegalovirus (CMV)promoter. The pred...
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In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...
متن کاملTransient Expression of Human Growth Hormone in Potato (Solanum tuberosum), Tobacco (Nicotiana tobacum) and Lettuce (Lactuca sativa) Leaves by Agroinfiltration
Using agro-infiltration technique, we have transiently expressed human Growth Hormone (hGH) in tobacco(Nicotiana tobacum), potato (Solanum tuberosum) and lettuce (Lactuca sativa) leaves. Out of three differentinoculation times used for infiltration in our study, it was seen that highest level of hGH expression wasachieved when leaves were infiltrated for 35 min. The presence o...
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ژورنال
عنوان ژورنال: Journal of Zhejiang University SCIENCE B
سال: 2008
ISSN: 1673-1581,1862-1783
DOI: 10.1631/jzus.b0710596